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| Menezes,Carlos C.E; Sediyama,Tocio; McDonald,Miller B; Dias,Denise C.F.S. |
| A técnica "Random Amplified Polymorphic DNA" (RAPD) surgiu como uma ferramenta útil para testar a pureza genética e a discriminação de cultivares em muitas espécies. É uma técnica simples, rápida, relativamente de baixo custo e permite o uso de DNA extraído de sementes secas, o que é muito importante em um programa de análise de sementes. O uso desta tecnologia no teste da pureza genética pode ser muito interessante para algumas espécies, como a vinca (Catharanthus roseus (L.) G.Don), pois pouco se conhece a respeito da seqüência de seu DNA. É interessante, também, pelo fato de existir grande número de "primers" comercialmente disponíveis, que podem ser prontamente utilizados para gerar dados. Essa técnica pode ser mais facilmente utilizada para gerar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vinca; Sementes; Variedades; Marcadores moleculares. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100039 |
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| Peñaloza,Patricia; Ramirez-Rosales,Gerardo; McDonald,Miller B; Bennett,Mark A.. |
| High seed quality is essential for optimum stand establishment in lettuce. As a result, it is necessary to have seed vigour tests that permit rapid, objective and accurate evaluation of seed quality. This study evaluated physical and physiological seed quality components of four seed lots of six lettuce varieties obtained from a commercial company. Seeds were evaluated for seedling emergence under greenhouse conditions, standard germination, seed physical aspects, the Saturated Salt Accelerated Aging (SSAA) test and the Seed Vigour Imaging System (SVIS). Results indicated that large-seeded lettuce varieties had higher percentage germination, higher SSAA values, higher SVIS index and more rapid and uniform greenhouse emergence. Black-seeded lettuce... |
| Tipo: Journal article |
Palavras-chave: Vegetables; Deterioration; Seedling; Performance. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300010 |
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| Menezes,Carlos C.E; Vantoai,Tara; McDonald,Miller B; Sediyama,Tocio. |
| Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vinca; Seed; Variety discrimination; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100041 |
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